Substrate specificity of Bacillus .ALPHA.-D-xylosidase.
نویسندگان
چکیده
منابع مشابه
Dual substrate specificity of Bacillus subtilis PBP4a.
Bacterial dd-peptidases are the targets of the β-lactam antibiotics. The sharp increase in bacterial resistance toward these antibiotics in recent years has stimulated the search for non-β-lactam alternatives. The substrates of dd-peptidases are elements of peptidoglycan from bacterial cell walls. Attempts to base dd-peptidase inhibitor design on peptidoglycan structure, however, have not been ...
متن کاملSubstrate Specificity of d Ribozyme Cleavage
The specificity of d ribozyme cleavage was investigated using a trans-acting antigenomic d ribozyme. Under single turnover conditions, the wild type ribozyme cleaved the 11-mer ribonucleotide substrate with a rate constant of 0.34 min, an apparent Km of 17.9 nM and an apparent second-order rate constant of 1.89 3 10 min M. The substrate specificity of the d ribozyme was thoroughly investigated ...
متن کاملVariation in relative substrate specificity of bifunctional β-D-xylosidase/α-L-arabinofuranosidase by single-site mutations: Roles of substrate distortion and recognition
To probe differential control of substrate specificities for 4-nitrophenyl-α-L-arabinofuranoside (4NPA) and 4-nitrophenyl-β-D-xylopyranoside (4NPX), residues of the glycone binding pocket of GH43 β-D-xylosidase/α-L-arabinofuranosidase from Selenomonas ruminantium were individually mutated to alanine. Although their individual substrate specificities (kcat/Km) 4NPX and (kcat/Km) 4NPA are lowered...
متن کاملEffects of substrate binding site residue substitutions of xynA from Bacillus amyloliquefaciens on substrate specificity
BACKGROUND The aromatic residues of xylanase enzyme, W187, Y124, W144, Y128 and W63 of substrate binding pocket from Bacillus amyloliquefaciens were investigated for their role in substrate binding by homology modelling and sequence analysis. These residues are highly conserved and play an important role in substrate binding through steric hindrance. The substitution of these residues with alan...
متن کاملPhoto-affinity Labeling of B-d-xylosidase
As part of a comparative study of the structure and function of a group of glycosidases, we are particularly interested in the properties of a P-Dxylosidase (/3-D-xyloside xylohydrolase, EC 3.2.1.37) induced in Bacillus pumilus [l] . In an attempt to obtain further information on the catalytic site of this enzyme, several potential affinity-labels have been prepared [2-41 and tested on this enz...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.53.2129